Assembly and Transcription of Chromatin Templates Using RNA Polymerase III
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The use of in vitro model systems has been essential to the dissection of the mechanisms for transcription regulation. It is now possible to reconstitute specific transcription by each of the three eukaryotic RNA polymerases using purified or at least partially purified protein factors (1 –3 ). In addition, many trunsacting factors have been shown to enhance in vitro transcription of target genes containing the appropriate cis-elements upstream of their promoters (4 –6 ).
