Differential Screening of Differential Display cDNA Products by Reverse Northern
互联网
432
Differential Display (DD) is a widely used methodology for cloning differentially expressed genes. Since its inception in 1992 (1 ), much effort has been made to improve and optimize the technique (2 ,3 ). One major bottle-neck remains, though, in the screening step for the verification of the cDNA fragments tentatively identified by differential display.
