Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler
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Recent developments in PCR fluorimetry have allowed for quick quantification
of target molecules. Before the invention of fluorimetric quantitative PCR, researchers who wanted to quantify the amount of a gene in a sample did so painstakingly by limiting dilution, competitive polymerase chain reaction (PCR), or other methods, including high-performance liquid chromatography (HPLC), solid phase assays, dot blots, or immunoassay (1 ).
