Identification of Phytophthora fragariae var. rubi by PCR

The following chapter describes a PCR method for the identification of the raspberry root rot pathogen Phytophthora fragariae var. rubi . Furthermore, a nested PCR suitable for the detection of the pathogen in infected raspberry roots and validated against the “Duncan bait test” (EPPO Bull 35:87–91, 2005) is explained. Protocols for different DNA extraction methods are given which can be transferred to other fungal pathogens.