Sequencing PCR Products Cloned into M13 Vectors
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The polymerase chain reaction (PCR) facilitates the rapid in vitro amplification of target DNA segments. Numerous applications have been developed to exploit the vast potential of PCR, and many of these require sequence analysis of the DNA product. This chapter describes the dideoxy chain-termination method for sequencing PCR products cloned into M13 and plasmid vectors.
