Purification Through Affinity Chromatography and Microsequencing

Production of monoclonal antibodies (MAbs) using adhesion-involved cell types as a source of antigens allows the identification of new molecules participating in the adhesion process (1 –3 ). Immunoaffinity purification followed by protein microsequencing is one of the techniques used to characterize the new adhesion molecule identified and to determine at least in part its amino acidic sequence (4 ,5 ).