Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production

Viral transduction of eukaryotic cell lines is one possibility to efficiently generate recombinant proteins, and among all viral-based expression systems the baculovirus/insect cell expression system (BEVS) is certainly the most well known and applied. This chapter delineates the individual process steps of the system: maintenance of insect cell cultures, transfection of recombinant DNA into different insect cell lines, amplification of virus stocks, virus titer determination, and accessory experiments required to give rise to optimal production conditions.