Lateral Diffusion Coefficients of Raft Lipids From Pulsed Field Gradient NMR
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The pulsed field gradient-nuclear magnetic resonance diffusion technique has an appreciable potential for biophysical investigations
in membrane biology, various lyotropic liquid crystals, and other complex fluid systems. In particular, topics like transport
of molecules both across and within the plane of a lipid membrane can be successfully studied, as well as the formation of
lipid domains and their intrinsic dynamics. The pulsed field gradient-nuclear magnetic resonance technique and the preparation
of oriented samples for investigations of lipid lateral diffusion in macroscopically aligned bilayers, oriented by a goniometer
probe in the main magnetic field, are described. Some recent results illustrating the potential of the method in detecting
and characterizing domain formation are also presented.
