• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Probing Antibody-Antigen Interactions by Mass Spectrometry

        互联网

        2646
        Techniques that have been used for antigenic site mapping of linear epitopes in proteins include binding assays of protein components produced by synthetic chemistry (1 ,2 ) or by recombinant gene expression (3 ,4 ). More recently, epitope localization has been achieved through the use of synthetic and bacteriophage peptide libraries (5 9 ). Although effective, these methods can be costly and time-consuming. A different approach to antigenic site mapping has been reported by Suckau (10 ), who compared the pattern of proteolytic digestion of free peptide antigen with the pattern produced from the antigen bound to an antibody. Alternatively, these workers subjected the peptide to proteolytic digestion and identified products that bound specifically to the immobilized antibody. In both cases, the peptides of interest were identified by 252 Cf plasma desorption mass spectrometry. Recently, another approach, termed affinity-directed mass spectrometry (11 ) has been reported by us and others (12 16 ) for probing antigen-antibody interactions.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序