• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Protein-Protein Interactions

        互联网

        1397
        Most proteins need to interact physically with other proteins to be able to perform their biological functions; the identification of new protein-binding partners for a protein of interest may therefore be a key strategy in defining the mechanism of action for a particular protein. Many different approaches to studying protein-protein interactions exist, but identifying which interactions are biologically significant is not trivial. Thus, once an interaction is found, the first step is to validate the suggested interactions using genetic, biochemical, and cell-biological approaches. Here we will focus on three complementary protein interaction approaches: (a) the yeast two-hybrid (YTH) system, a genetic assay performed in living yeast cells, which can be used both to identify proteins that bind to a protein of interest and to determine domains or residues critical for an interaction; and two biochemical approaches, (b) the coimmunoprecipitation of interacting proteins with a specific antibody; and (c) the far-Western method that can show direct physical interaction of two proteins.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序