Quantitative RT-PCR from Fixed Paraffin-Embedded Tissues by Capillary Electrophoresis

It is possible to analyze RNA extracted from paraffin embedded-tissues that have been fixed in formalin (1 -3 ). In fact, all biopsy or surgical tissues are routinely fixed and paraffin-embedded and conserved for many years for future examination, and present a very valuable medical and research resource for epidemiological studies, and for the development of public health policies. The specific analysis of RNA remaining embedded in carefully preserved tissues facilitates the utilization of human pathological tissue samples for such diagnostic purposes.