• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Cell-Free Ion-Channel Recording

        互联网

        882
        When membrane patches are isolated from cells, one gains the ability to regulate precisely the composition of the solution bathing both surfaces of the membrane and to change the composition rapidly. However, in detaching the membrane from the underlying cytoskeleton, one irreversibly changes the integrated system of which the channel protein is a part, and begins an inexorable loss of channel function. In this chapter we will deal primarily with the techniques available for measuring the response of patch currents to changes in bathing solution and the practical problems to be minimized, or overcome, in implementing such techniques and analyzing the results obtained. Other chapters will deal with patch-clamping in general. Chapter 6 deals specifically with methods of changing the concentration of the solution bathing the intrapipet face of the membrane, and we will concentrate on consideration of the extrapipet solution changes, i.e., changes at the intracellular membrane surface in inside-out patches, or the external face in outside-out membrane patches.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序