Small-Scale Plasmid DNA Preparation
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		For the initial characterization of a recombinant plasmid, it is necessary to determine the size of the plasmid or, preferably, the size and characteristics of the insert itself. A method is therefore required for the simultaneous preparation, from a number of isolates, of plasmid DNA in a state sufficiently pure for restriction enzyme digestion. The requirements of such a procedure are:
		
			
				
	
| (i) | 
							A simple method for rapid lysis of the bacterial cells. | 
| (ii) | 
							Separation of plasmid from chromosomal DNA. | 
| (iii) | 
							Removal of proteins and of other components of the cells that might interfere with restriction enzyme treatment. | 
| (iv) | 
							Removal of detergents, salts, etc. used in the process. | 











