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        酵母转化

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        3094

         

                 Yeast Transformation (Gietz Lab)
        LiAc/SS-DNA/PEG Transformation

                 Yeast Transformation (Breeden Lab)
        LiAc method

                 Large-Scale Yeast Transformation (Yale Yeast Analysis Center)
        Detailed protocol for large-scale yeast transformation including competent cell preparation, transfromation...

                 Yeast Transformation ( Herskowitz Lab)

                 Sleazy Plate Transformation of Yeast ( Herskowitz Lab)

                 Transformation of Yeast - LiAc Method (Lazo Lab)  

                 Two Hybrid System Transformation (Gietz Lab)
        This  Protocol is for 2 Hybrid system screens

                 Yeast Transformation (Fangman/Brewer lab)
        Yeast transformation protocol  Using Frozen Competent Cells and Single-stranded DNA as a Carrier

                 Quick Yeast Transformation (Gietz Lab)
        This Protocol allows for transformation with any Yeast cell source. When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. This procedure is very flexible and can be applied to yeast cells from a number of different sources; however, for best results, use cells from a freshly grown plate.

                 Yeast Transformation (D. Amberg)
        Electroporation method

                 Fission Yeast Transformation (Forsburg Lab) 
        Includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion. 

                 Multiwell Transformation  (Davis Lab)
        Detailed protocol with LiAcetate PEG transformation method

                 Transformation of Yeast (PMCI Research)

                 "Lazy Bones" Plasmid Transformation (Dohlman Lab) 
        A quick method for plasmid transformation into yeast.

                 Competent Yeast Preparation (Gietz Lab)
        This Protocol allows you to prepare frozen yeast cells that are competent for transformation after thawing

                 Yeast Transformation (Gottschling Lab)
        Provides LiAc method, "lazy bones" method and electroporation method 

                 Yeast Transformation (Finley Lab) 
        LiOAc method

        • Yeast Transformation with DMSO/LioAc (high efficiency) (Hahn Lab)
            
        • Yeast Transformation (Rapid method) (Hahn Lab)
          LiOAc method

                 Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue (Gottschling Lab)

                 Yeast Colony PCR (Gottschling Lab)
        For yeast transformant screening

                 Direct PCR from Whole Yeast Cells (Namjin Chung)
        Zymolyase Method for screening yeast transformants

         

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