• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构：
    • 成为企业机构
    • 个人用户：
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Measurement of Agonist-Stimulated [35S]GTPS Binding to Cell Membranes

        互联网

        549
        This chapter describes a functional assay which measures the increase in guanine nucleotide exchange at G-proteins in cell membranes, resulting from agonist binding to G-protein-coupled receptors (GPCRs), by monitoring the binding of [35 S]GTPγS, a radiolabeled, hydrolysis-resistant analog of GTP (guanosine triphosphate), in the presence of unlabeled GDP (guanosine triphosphate).
        ad image
        ad image

        相关产品推荐

        Hemagglutinin/HA重组蛋白|Recombinant H1N1 (A/California/04/2009) HA-specific B cell probe (His Tag)

        Hemagglutinin/HA重组蛋白|Recombinant H1N1 (A/California/04/2009) HA-specific B cell probe (His Tag)

        ￥2570

        Cortisol Binding Globulin/CBG重组蛋白|Recombinant Human SerpinA6 Protein (His & AVI Tag), Biotinylated

        Cortisol Binding Globulin/CBG重组蛋白|Recombinant Human SerpinA6 Protein (His & AVI Tag), Biotinylated

        ￥2980

        RORγt agonist 3，2664106-24-3，≥98%，阿拉丁

        RORγt agonist 3，2664106-24-3，≥98%，阿拉丁

        ￥1600.90

        pCT-CMV-PSD95-GFP（pCMV，Dendrite Membranes，PSD-95 Tag）-pCT-CMV-PSD95-GFP (pCMV, Dendrite Membranes, PSD-95 Tag)

        pCT-CMV-PSD95-GFP（pCMV，Dendrite Membranes，PSD-95 Tag）-pCT-CMV-PSD95-GFP (pCMV, Dendrite Membranes, PSD-95 Tag)

        ￥55

        [35S]－Met细胞代谢标记试剂盒

        [35S]－Met细胞代谢标记试剂盒

        ￥1100

        相关问答

        问

        磁珠分选小鼠CD3+T cell

        3 回答 855 围观
        2023-06-17

        问

        Cell reports投稿难度如何？

        2 回答 1975 围观
        2022-10-14

        问

        小鼠效应T和记忆T cell

        1 回答 2216 围观
        2022-03-04

        相关方法

        用[35S]蛋氨酸和[35S]半胱氨酸标记哺乳动物细胞

        推荐阅读

        In Vitro Autoradiographic Localization of Receptor-Stimulated [35S]GTPS Binding in Brain

        [35S]GTPS Binding in G Protein-Coupled Receptor Assays

        Measurement of Agonist-Stimulated [35S]GTPγS Binding to Assess Total G-Protein and Gα-Subtype-Specific Activation by G-Protein-Coupled Receptors

        无忧采购 轻松科研

        无忧采购 轻松科研

        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序