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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海中乔新舟生物科技有限公司
- 规格:
1 ml
| 货号 |
CSP139 |
| 名称 |
细胞包被液(适合原代细胞) (Cell adherent reagent) (200x) |
| 描述 |
本款产品是一种新型化合物,用之室温处理培养瓶30分钟,能够使原代或传代培养细胞的粘附贴壁能力显著增加,处理效果优于使用多聚赖氨酸和胶原蛋白,对绝大多细胞类型均有明显效 果,本产品无细胞毒性,不影响后续免疫荧光组化和酶学测定等实验,适用于各种原代细胞,如乳鼠脑神经元,易漂浮的传代细胞,如PC12和HEK293细胞,也适用于药物处理易漂浮细胞;不仅能够明显改善细胞生长状态、增加细胞数量,还可以使细胞容易耐受洗涤、换液、药物处理造成的脱落。对于生长在处理过的瓶皿中的细胞,使用细胞贴壁粘附剂可使细胞的转染效率显著增加2-5倍。 |
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文献和实验论文标题: NU9056, a KAT 5 Inhibitor, Treatment Alleviates Brain Dysfunction by Inhibiting NLRP3 Inflammasome Activation, Affecting Gut Microbiota, and Derived Metabolites in LPS-Treated Mice
DOI: 10.3389/fnut.2021.701760
发表时间: 2021-07-13
期刊: Frontiers in Nutrition
影响因子: 6.576
货号: ZQ0397
产品名称: BV2 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8313765/
4. Add the pre-warmed dissociation reagent such as trypsin or TrypLE™ to the side of the flask; use enough reagent to cover the cell layer (approximately 0.5 mL per 10 cm2 ). Gently rock the container to get complete coverage of the cell layer
Immunostaining of Cells Adherent to Coverslips
and 2x 100 EtOH, then immerse in xylene (in chemical hood; all 1 min each). Place cover slip cell layer down on a glass slide containing a drop of mounting medium and examine in the light microscope. Reagent:
Procedure for fixing and staining adherent cells for immunofluorescence
Description This procedure describes how to process cells for immunofluoresence microscopy. Procedure 1. Sterilize coverslips under UV light for 20-30m a. To increase cell adherence, treat coverslips with a 1:10 dilution of poly-lysine
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