Quantitative Proteomic Analysis of Mammalian Organisms Using Metabolically Labeled Tissues
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Metabolic labeling of mammalian organisms with stable isotopes can be used to provide tissue-specific internal standards for
use in quantitative proteomic analyses. This method provides an alternative and complementary strategy to covalent modification
approaches using isotope-coded mass tags. This chapter will focus on the generation of the isotope-labeled tissues, the analysis
of the sample using Multidimensional Protein Identification Technology, and the computational analysis of the mass spectrometric
data acquired.
