Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens

The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation because of its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of one’s antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.