Use of cDNA Library Expression Cloning to Identify Components of Heterodimeric Receptor Complexes

Expression cloning in mammalian host cells has revolutionized immunology. The current protocol was originally developed by Dr. Brian Seed in 1987 (1 ), and it has gone through a number of improvements over the years. The most important feature of this technique has been to exploit the exquisite specificity of antibodies for antigens by developing a method permitting gene cloning in a mammalian cell system.