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        基因敲除--Gene Knockout Protocols

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        1699
        基因敲除--Gene Knockout Protocols
         
        Methods in Molecular Biology VOLUME158
        Gene Knockout Protocols 基因敲除实验方法
        M. J. Tymms and I. Kola
        Humana Press Inc.

         PDF格式,共437页,4.5M,点击此处下载

         内容:
        1  Overview  Gene Knockouts
        2  Isolation and Maintenance of Primate Embryonic Stem Cells
        3  Gene Targeting in ES Cells
        4  Manipulating Mouse Embryonic Stem Cells
        5  Gene Targeting in a Centralized Facility
        6  The LoxP/CRE System and Genome Modification
        7  Creation and Use of a Cre Recombinase Transgenic Database
        8  Choice of Mouse Strains for Gene Targeting
        9  Isolation, Microinjection, and Transfer of Mouse Blastocysts
        10 Aggregation Chimeras
        11 How to Study Pathologic Phenotypes of Knockout Mice
        12 Analysis of Hematopoietic Phenotypes in Knockout Mouse Models
        13 Isolation of Embryonic Fibroblasts and Their Use in the In Vitro
            Characterization of Gene Function
        14  Influence of Genetic Background on Knockout Mouse Phenotypes
        15 Lineage Allocation During Early Embryogenesis
        16 Generation of Double-Knockout Embryonic Stem Cells
        17 In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes
        18 Embryonic Stem Cells in the Study of Hematopoiesis
        19 Interferon-Inducible ES Cell Expression Systems
        …………
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