End-Rescue of YAC Clone Inserts by Inverse PCR
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The termini of yeast artificial chromosome (YAC) inserts can serve as probes or sequence tagged sites (STSs) that are useful in genomic analysis. For example, these elements can be used to:
| 1. |
Build physical maps and YAC contigs;
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| 2. |
Characterize and orient YAC clones;
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| 3. |
Assess chromosomal origin and YAC chimerism; and
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| 4. |
Construct conventional and rare-cutting restriction maps.
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