标准PCR

互联网2013-09-06

2214

・ What's PCR? (Michael Blaber's Lab)
Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not only for those new to PCR, but also those familiar with PCR.

・ PCR Guide (Michael Blaber's Lab)
Practical and useful guide to selection of polymerase, primer design, and more...

・ PCR Guide (Qiagen)
General guide to primer design, degenerate primer design and long PCR cycling conditions.

・ Standard PCR (Biotech)
Detailed PCR protocol including preparation of the sample, the master mix and the primers, detection and analysis of the reaction products.

・ PCR Guide (LaboratoryExperiments.com)
Basic guide high school students, But very useful if you are new to molecular biology.

・ PCR (Julie B. Wolf,UMBC)
PCR components and cycling conditions

・ PCR and multiplex PCR guide (Tavi)
Detailed guide for PCR optimization, like concentration of usr/localious components, cycling conditions, adjuvants and more.

・ Standard PCR Protocol (Molecular Biology Techniques Manual)
The followings are described in detail
Recommended Reagent Concentrations
Recommended Reaction Conditions
"Hot Start" PCR
Asymmetric PCR for ssDNA Production
Detecting Products
Labeling PCR Products with Digoxigenin
Cleaning PCR Products
Sequencing PCR Products
Cloning PCR Products

・ PCR (Bowtell Lab)

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Standard PCR


 (Chen)

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Standard PCR, primer design, cycling, additives and more

・ PCR (Fermentas)
Give detailed general protocol for PCR, including preparing reaction mixture, cycling conditions, guidelines to avoid contamination and more...

・ PCR (Dr. Chastain)
PCR components and setting up PCR reaction.

PCR Manual (BMB)
This manual contains detailed protocols on performing PCR as well as preparation of templates and post-PCR clean-up. Important applications such as PRINS, cycle sequencing, detection of human chromosomes, and non-radioactive labeling are discussed.

・ Amplification of Genomic DNA by PCR (Robert H. Cruickshank)
Standard and detailed PCR protocol with trouble shooting guide

・ PCR Program Design (Tavi)

・ PCR Trouble Shooting (Alkami Biosystems)
Very useful check points for PCR trouble shooting

・ Calculating Concentrations for PCR (Molecular Biology Techniques Manual)
How to calculate primer and nucleotide concentration

・ PCR in A Teacup (ktotah@sidwell.edu )
PCR in teacup can simply be defined as PCR without a PCR machine. This "old-fashioned" method uses hot plates and water baths to go through the amplification process. Because of its tediousness it is not the most successful way to perform a polymerase chain reaction, but its advantages are that it is inexpensive and simple.

・ Detect Alu repeat by PCR (Biotech)
PCR is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu within the tissue plasminogen activator (TPA) gene.

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