Tween 80的性质及实验室应用注意事项（不宜高温灭

分子式：C64 H124 O26

分子量：1310 g/mol

外表：Amber colored viscous liquid

密度：1.06-1.09 g/mL, oily liquid

学名：聚氧乙烯山梨糖醇酐单油酸酯，又名T-80乳化剂

别名: Polysorbate 80, PEG (80) sorbitan monooleate, polyoxyethylenesorbitan monooleate

形成微球的分子量: 76 kDa

制备：山梨糖醇酐单油酸酯和氧化乙烯反应制得

生物学实验中应用

TWEEN 80广泛应用于生化实验当中，包括①稳定蛋白构象；②从培养基培养的细胞中分离细胞核；③培养杆菌，如分枝杆菌；④用作乳化剂。

TWEEN 80在冰激淋加工中的作用

Tween 80 is often used in ice cream to prevent milk proteins from completely coating the fat droplets. This allows them to join together in chains and nets, to hold air in the mixture, and provide a firmer texture, holding its shape as the ice cream melts.

用Tween 80配制溶液

TWEEN 80 is miscible in water (0.1 ml/ml) yielding a clear to slightly hazy faint yellow solution. It is reported to be miscible with alcohol, cottonseed oil, corn oil, ethyl acetate, methanol, and toluene, but insoluble in mineral oil.

The pH of a 1% aqueous solution is 5.5-7.2.

稳定性及保存

Aqueous solutions of polysorbates as well as the neat liquid will undergo autoxidation over time, with changes being catalyzed by light, increased temperature, and copper sulfate. （Tween 80易发生自氧化 作用，应避光 、低温保存）

Solutions are reasonably stable at 2 - 8 ℃ for short periods.

For special applications, storage under argon or nitrogen may be preferred.

Sigma的产品Tween 80并非无菌的. Sigma建议用0.22微粒孔径的滤膜过滤除菌，不建议采用高温灭菌的方式。

(责任编辑：大汉昆仑王)