Fluorescence-Based Fragment Size Analysis

The successful application of capillary electrophoresis technology to the genotyping of various types of polymorphisms has been well documented. The flexibility and automation of the Applied Biosystems 3100 Genetic Analyzer make it an excellent capillary electrophoresis platform for the generation of high quality genotype data. These data are readily applied to pharmacogenomic investigations of various types. Included in this chapter is a protocol for the generation of genotype data using minimal template deoxyribonucleic acid and maximizing the automation of both data analysis and genotype assignment through the use of the Applied Biosystems GeneMapper 3.0 software package.