Fluorescence-Activated Cell Sorting According to Receptor Density: Application for Isolating Transfected Cell Lines
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Isolation of cells expressing transfected cDNAcan be extremely difficult and tedious if the efficiency of expression is low (less than 1–5%). If the receptor being selected for is a cell surface or integral membrane protein at least partly exposed to the exterior, then it is possible to use the extremely powerful technique of fluorescence-activated cell sorting (FACS) to select these cells from a receptor-negative background.
