• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        A Rapid Plasmid Purification Method for Dideoxy Sequencing

        互联网

        549
        The dideoxy chain-termination method of DNA sequence analysis involves the synthesis of a DNA strand from a single-stranded template (1 ). The enzymatic synthesis is initiated at the site where an oligonucleotide primer anneals to the template. Traditionally, sequencing was performed on templates produced by single-stranded phage Ml3 (2 ,3 ), or phagmid (4 ). Sequencing plasmid DNA has the advantage of eliminating the need to subclone fragments into M13, and also enables both strands to be sequenced from the same plasmid by the use of reverse primers. The most consistently satisfactory sequencing results for most purposes are probably obtained by using linear amplification techniques (cycle sequencing) either in manual procedures (5 ) or in automated sequencing machines (6 ). However, plasmid DNA that has been denatured can also serve as satisfactory template DNA for manual, nonamplified procedures (7 ). It is particularly important when preparing plasmid DNA for sequencing to give the utmost care and attention to the DNA isolation and purification techniques. Most problems that occur with plasmid sequencing are related to poor quality template.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序