Silica Clean-up of DNA
互联网
726
<center> <h1> Silica Clean-up of DNA</h1> </center>
Materials:
|
Silica Suspension: |
add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend in 2 vol H2O |
|
Silica Wash Solution: |
50 mM NaCl, 10 mM Tris 7.5, 2.5 mM EDTA, 50% Ethanol |
|
6 M NaI |
note: light sensitive |
