Affinity Measurements with Radiolabeled Antibodies

The use of radioiodinated proteins is one of the most sensitive methods to demonstrate and quantify specific protein/protein interactions. The direct introduction of Iodine-125 into proteins is easily and quickly done, provided the mandatory safety equipment is available. Most antibodies can be radioiodinated to high specific radioactivity, although each individual antibody must be characterized regarding its optimal compromise between specific radioactivity and bioactivity.