A Method for Determination of UDP-GlcNAc: GlcNAc1-6(GlcNAc1-2)Man1-R [GlcNAc to Man] 1-4N-Acetylglucosaminyltransferase VI Activity
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To characterize and purify glycosyltransferases, it is essential to establish a simple and sensitive assay method. Here, we describe a method for determination of the activity of GnT VI (UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N -acetylglucosaminyltransferase VI) using a fluorescently labeled oligosaccharide.
