Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
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The dynamic addition of O -GlcNAc to target proteins is now recognized as a major signaling paradigm impacting phosphorylation, protein turnover, gene expression, and other posttranslational modifications influencing epigenetics. Here we describe the production of and methods for assay of the recombinant enzymes of O -GlcNAc cycling: O -linked GlcNAc Transferase (OGT) and O -GlcNAcase (OGA).
