PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles
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930
		The use of molecular typing methods for defining human leukocyte antigen (HLA) Class I and Class II alleles is now commonplace. Molecular methods offer a greater accuracy than traditional serological methods (1  –5  ) which is reflected in many disease and transplantation studies (6  –9  ). This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10  ,11  ) . PCR-SSP is also variably known as allele-specific PCR or amplification refractory mutation system (ARMS).











