- ￥1350 - 3000
- Proteintech
- 66013-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, FC, ELISA
- Mouse
- human, pig
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HLA class I (HLA-A) protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, pig
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC, ELISA
- 靶点：
HLA class I ABC
- 抗体英文名：
HLA class I ABC Monoclonal antibody
- 抗体名：
HLA class I ABC Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human white blood, NCCIT cells, pig spleen tissue, U-937 cells, Raji cells, HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
| Positive FC detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66013-1-Ig targets HLA class I ABC in WB, IHC, IF/ICC, FC, IP, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, FC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag7488 Product name: Recombinant human HLA class I (HLA-A) protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 28-303 aa of BC003069 Sequence: SMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQ |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class I, A |
| 别名 | HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A |
| 计算分子量 | 41 kDa |
| 观测分子量 | 40-44 kDa |
| GenBank蛋白编号 | BC003069 |
| 基因名称 | HLA-A |
| Gene ID (NCBI) | 3105 |
| RRID | AB_11042593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04439 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
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文献和实验Binding of Soluble KIR-Fc Fusion Proteins to HLA Class I
a precise ligand specificity to any given receptor on an NK cell. This difficulty can be circumvented by using an assay that detects direct binding of soluble forms of individual KIR to transfected cell lines that express single HLA class I allotypes.
一. HLA-Ⅰ类分子的分布、结构和功能 1. 分布： 有核细胞(含血小板和网织红细胞)表面。 存在形式： 膜结合：不同的组织细胞表达Ⅰ类分子量不同，如：淋巴细胞(最多)，肾、肝脏及心脏 (其次)，神经组织(很少) 。 可溶性：存在于血清、初乳和尿液等体液中。 2. 结构： 二条多肽链 (非共价键连接的糖蛋白, Ig超家族成员)： ◆ a链（重链）： 340个氨基酸残基，分子量44 kDa，MHC-Ⅰ类基因编码，具有高度多态性。 胞外区--- a1、a2
Immunology.lst ed Philadelphia:W.B.Saunders Company,1991;98-114 2.Bjorkman PJ,et al.Structure of the human class i histocompatibility antige,HLA-A2.Nature,1987;329:506 3.Davis M,Bjorkman PM.T-cell antigen receptor genes and T-cell recognition.Nature
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