Mouse Models of Bariatric Surgery
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- Abstract
- Table of Contents
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- Literature Cited
Abstract
Morbid obesity is linked to increased incidence of glucose intolerance, Type 2 diabetes mellitus, cardiovascular diseases, various forms of liver disease, and specific forms of cancer. Treatment of obesity by lifestyle modifications (i.e., changes in diet and exercise) and drug therapy is generally ineffective. Bariatric surgery is currently the most effective means of treating obesity and related disorders. We as well as others have developed surgical procedures for application to genetic mouse models that mimic an array of human bariatric surgical procedures used in the treatment of obesity. The application of bariatric surgery to genetic mouse models will broaden our understanding of the role of the gut in metabolic disease. Models that have been developed include gastric banding, sleeve gastrectomy (SG), Roux?en?Y gastric bypass (RYGB) with a complete exclusion of the stomach, duodenal?jejunal bypass (DJB), and biliopancreatic diversion (BPD). The detailed methods of these procedures are provided. Curr. Protoc. Mouse Biol. 2:295?306 © 2012 by John Wiley & Sons, Inc.
Keywords: bariatric surgery; gastric banding; sleeve gastrectomy; Roux?en?Y gastric bypass; duodenal?jejunal bypass; biliopancreatic diversion; mice
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PDF or HTML at Wiley Online LibraryTable of Contents
- Introduction
- Basic Protocol 1: General Procedures and Care
- Basic Protocol 2: Gastric Banding
- Basic Protocol 3: Sleeve Gastrectomy (SG)
- Basic Protocol 4: Roux‐en‐Y Gastric Bypass (RYGB)
- Basic Protocol 5: Duodenal‐Jejunal Bypass (DJB)
- Alternate Protocol 1: Biliopancreatic Diversion
- Commentary
- Literature Cited
- Figures
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Basic Protocol 1: General Procedures and Care
Materials
Basic Protocol 2: Gastric Banding
Materials
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Figure 1. Gastric banding. (A ) Illustration of gastric banding. (B ) Photo of gastric banding. View Image -
Figure 2. Sleeve gastrectomy. (A ) Illustration of sleeve gastrectomy. The stomach is exposed and approximately 80% of the stomach is removed (the fore‐stomach and 70% of the granular stomach). (B ) Photo of sleeve gastrectomy. View Image -
Figure 3. Roux‐en‐Y gastric bypass (RYGB). (A ) Illustration of RYGB with complete exclusion of the stomach. The mouse RYGB involves the anastomosis between the esophagus and the jejunum, which is different from the RYGB procedure performed in humans that creates a stomach pouch out of a small portion of the stomach and attaching it directly to the small intestine. The mouse RYGB bypasses the whole stomach and duodenum. E‐J, esophago‐jejunal anastomosis; JJ, jejuno‐jejunal anastomosis. (B ) Illustration of RYGB. View Image -
Figure 4. Duodenal‐jejunal bypass (DJB). (A ) Illustration of DJB. DJB consists of a stomach‐preserving bypass of the duodenum and a short segment of the proximal jejunum. The distal jejunum is anastomosed to the proximal duodenum. D‐J, duodenal‐jejunal anastomosis; J‐J, jejuno‐jejunal anastomosis. (B ) Photo of DJB. View Image
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Literature Cited
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