General Considerations for Mouse Survival Surgery
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- Abstract
- Table of Contents
- Figures
- Literature Cited
Abstract
Personnel performing surgery on mice commonly lack formal medical training and most training in mouse surgery focuses on the mechanical skills required for a procedure with less emphasis on perioperative techniques. Consequently, a basic concept that underpins successful surgery, aseptic technique, may not be fully understood or incorporated into the training process. This unit provides a framework in which to plan and carry out surgical procedures in mice using appropriate aseptic technique. Curr. Protoc. Mouse Biol. 2:263?271 © 2012 by John Wiley & Sons, Inc.
Keywords: surgery; aseptic; mouse
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PDF or HTML at Wiley Online LibraryTable of Contents
- Introduction
- Aseptic Surgical Technique
- Presurgical Preparation
- Intraoperative Procedures
- Postoperative Recovery
- Conclusion
- Literature Cited
- Figures
- Tables
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Figure 1. Example of equipment and instrument set up for mouse surgery. Circulating water blanket (A ); clear surgical drape overlapping the sterile surgery field (B ); sterile field (C ); stereomicroscope (D ); goose neck lamp (E ); sterile saline for removing debris from instrument tips (F ); glass bead sterilizer (G ); and instruments cooling after the tips were sterilized in a glass bead sterilizer (H ). View Image
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Literature Cited
| AWA. 2010. Animal Welfare Act. United States Department of Agriculture (USDA), National Agricultural Library. | |
| Bradfield, J.F., Schachtman, T.R., McLaughlin, R.M., and Steffen, E.K. 1992. Behavioral and physiologic effects of inapparent wound infection in rats. Lab. Anim. Sci. 42:572‐578. | |
| Clough, G. 1982. Environmental effects on animals used in biomedical research. Biol. Rev. Camb. Philos. Soc. 57:487‐523. | |
| Committee for the Update of the Guide for the Care and Use of Laboratory Animals, Division on Earth and Life Studies. 2011. Guide for the Care and Use of Laboratory Animals, Eighth ed. The National Academies Press, Washington, D.C. | |
| Foreman, O., Kavirayani, A.M., Griffey, S.M., Reader, R., and Shultz, L.D. 2011. Opportunistic bacterial infections in breeding colonies of the NSG mouse strain. Vet. Pathol. 48:495‐499. | |
| Hostler, D., Zhou, J., Tortorici, M.A., Bies, R.R., Rittenberger, J.C., Empey, P.E., Kochanek, P.M., Callaway, C.W., and Poloyac, S.M. 2010. Mild hypothermia alters midazolam pharmacokinetics in normal healthy volunteers. Drug Metab. Dispos. 38:781‐788. | |
| The Jackson Laboratory, 2012. Workshop on Surgical Techniques in the Laboratory Mouse. Bar Harbor, ME: The Jackson Laboratory. | |
| Keen, J.N., Austin, M., Huang, L.S., Messing, S., and Wyatt, J.D. 2010. Efficacy of soaking in 70% isopropyl alcohol on aerobic bacterial decontamination of surgical instruments and gloves for serial mouse laparotomies. J. Am. Assoc. Lab. Anim. Sci. 49:832‐837. | |
| PHS. 2002. Public Health Service Policy on Humane Care and Use of Laboratory Animals, Office of Laboratory Animal Welfare: Public Health Service Policy on Humane Care and Use of Laboratory Animals. Bethesda, Md. | |
| Raabe, B.M., Artwohl, J.E., Purcell, J.E., Lovaglio, J., and Fortman, J.D. 2011. Effects of weekly blood collection in C57BL/6 mice. J. Am. Assoc. Lab. Anim. Sci. 50:680‐685. | |
| Tortorici, M.A., Kochanek, P.M., and Poloyac, S.M. 2007. Effects of hypothermia on drug disposition, metabolism, and response: A focus of hypothermia‐mediated alterations on the cytochrome P450 enzyme system. Crit. Care Med. 35:2196‐2204. | |
| Internet Resources | |
| http://courses.jax.org/event‐listings.html | |
| The Jackson Laboratory's Web site for the Workshop on Surgical Techniques in the Laboratory Mouse. | |
| http://awic.nal.usda.gov/government‐and‐professional‐resources/federal‐laws/animal‐welfare‐act | |
| Animal Welfare Act. United States Department of Agriculture (USDA), National Agricultural Library. | |
| http://grants.nih.gov/grants/olaw/references/phspol.htm | |
| Public Health Service Policy on Humane Care and Use of Laboratory Animals, Office of Laboratory Animal Welfare: Public Health Service Policy on Humane Care and Use of Laboratory Animals. | |
| http://www.nap.edu/catalog.php?record_id=12910 | |
| Committee for the Update of the Guide for the Care and Use of Laboratory Animals, Division on Earth and Life Studies. 2011. Guide for the Care and Use of Laboratory Animals, Eighth Edition. |
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