In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors
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In vitro transcription provides information not necessarily available from transfection assays about the regulatory activity of a promoter transcribed by RNA polymerase II. In the particular application discussed here, when an oligonucleotide duplex containing a recognition site for a specific transcription factor is included in the transcription reaction using nuclear extract, the factor can be selectively depleted from a pool of binding factors that are required to regulate transcription from the class II promoter.
