• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        关于核酸和蛋白的一些换算

        互联网

        2034

        一,Spectrophotometric Conversions
        1 A260 unit of double-stranded DNA=50 µg/ml
        1 A260 unit of single-stranded DNA=33 µg/ml
        1 A260 unit of single-stranded RNA=40 µg/ml

        二,DNA Molar Conversions
        1 µg of 1,000 bp DNA =1.52pmol(3.03pmol of ends)
        1 µg of pBR322 DNA =0.36pmol DNA
        1 pmol of 1,000 bp DNA =0.66 µg
        1 pmol of pBR322 DNA =2.8 µg

        三,Formulas for DNA Molar Conversions
        1,For dsDNA
        To convert pmol to µg: pmol × N × 660 pg/pmol × 1 µg/106pg = µg
        To convert µg to pmol: µg × 106pg/1 µg × pmol/660pg × 1/N = pmol
        2,For ssDNA
        To convert pmol to µg: pmol × N × 330 pg/pmol × 1µg/106pg = µg
        To convert µg to pmol: µg × 106pg/1 µg × pmol/330pg × 1/N = pmol
        #where N is the number of nucleotides and 330 pg/pmol is the average MW of a nucleotide

        四, Protein Molar Conversions
        100 pmol of 100 kDa protein = 10 µg
        100 pmol of 50 kDa protein = 5 µg
        100 pmol of 10 kDa protein = 1 µg
        100 pmol of 1 kDa protein = 100 ng

        五,Protein/DNA Conversions
        1 kb DNA = 333 amino acids= 37 kDa protein
        270 b DNA = 10 kDa protein
        810 b DNA = 30 kDa protein
        1.35 kb DNA = 50 kDa protein
        2.7 kb DNA = 100 kDa protein
        #average MW of an amino acid = 110 daltons
        #Dalton (Da) is an alternate name for the atomic mass unit, and kilodalton (kDa) is 1,000 daltons. Thus a protein with a mass of 64 kDa has a molecular weight of 64,000 grams per mole

        六,Agarose Gel(%):Resolution of Linear DNA
        Recommended % Agarose---- Optimum Resolution for Linear DNSize of fragments in nucleotides;bp
        0.5---------------1,000-30,000
        0.7--------------- 800-12,000
        1.0--------------- 500-10,000
        1.2--------------- 400-7,000
        1.5---------------200-3,000
        2.0----------------50-2,000

        七,Polyacrylamide Gel(%):Resolution of Protein
        Recommended % Acrylamide--------- Protein Size Range
        8 ---------------------- 40-200 kDa
        10 ---------------------- 21-100 kDa
        12----------------------- 10-40 kDa

        八,Length/M.W. of Common Nucleic Acids.
        Nucleic Acid ------Number of Nucleotides --------- Molecular Weight
        lambda DNA------ 48,502(dsDNA) ------------ 3.2 × 107
        pBR322DNA------ 4,361(dsDNA ------------ 2.8 × 106
        28S rRNA--------- 4,800 --------------- 1.6 × 106
        23S rRNA(E.coli)----- 2,900--------------- 1.0 × 106
        18S rRNA -------- 1,900 ---------------- 6.5 × 105
        16S rRNA(E.coli) ----- 1,500 -------------- 5.1 × 105
        5S rRNA(E.coli) ----- 120 ---------------- 4.1 × 104
        tRNA(E.coli)--------- 75 ---------------- 2.5 × 104
        #Molecular weights based on actual sequence.

        九,Stardards
        1. Average MW of dsDNA base pair = 600.
        2. Average MW of ssDNA base =330.
        3. Average MW of RNA base =340.[iframe][/iframe]

         

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序