• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Quick Isolation of DNA from Agarose Gels

        互联网

        906

        Hancock Laboratory Methods. Department of Microbiology and Immunology,

        University of British Columbia, British Columbia, Canada http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=67

        METHOD:

        1.Run gel as normal in 1X TBE.

        2.Visualize band under LONG wave UV.

        3.Cut band out with razor blade.

        4.Place excised agarose slice in an Eppendorf tube, which has a hole in its bottom (by inserting a hot needle), and which has a small amount of siliconized glass wool covering the opening.

        5.Place this tube inside another Eppendorf tube.

        6.Spin tubes either at 1/2 maximum speed for 15 minutes or, if not possible, full speed for 5 minutes.

        7.Phenol/chloroform the resulting liquid.

        8.Repeat with 1/10 volume 3 M NaAc 2x volume EtOH.

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序