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        请问有什么软件可以筛选出序列中的hairpin structure??

        丁香园论坛

        805
        就是在一篇文章中他们从一个序列数据库中筛选可能的miRNA,第一步是筛选可能的带hairpin的miRNA的precusor。他使用的是一个perl编辑的程序,可是肯定不会共享,看到其他的文章也是用了自己的程序,请问有没有免费的现成的程序可以用????
        文章在下面!!!

        The Arabidopsis genome version 3 and the O. sativa genome
        released by The Institute for Genome Research (TIGR) on 22
        July 2002 [49] and 24 January 2003 [50], respectively, were
        used for the present study. Intergenic regions of both the A.
        thaliana and the O. sativa genomes were extracted according
        to the annotations provided by TIGR. A scanning algorithm
        implemented in the Perl programming language was used to
        search for possible hairpin structures within each intergenic
        sequence in A. thaliana. The method inspects each successive
        21-nucleotide query window in each intergenic region, with
        two nucleotide increments, and searches for downstream
        complementary sequences with up to 25% mismatches. We
        restricted the distance from the last base of the forward query
        sequence and the first base of the downstream complementary
        pairing sequence to a minimum of 10 nucleotides and a
        maximum of 150 nucleotides (loop length). For
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