Quantification of Wound Angiogenesis

Angiogenesis occurs during embryonic development and in adult tissues (1 ). In developmental angiogenesis, the primitive vascular network formed during neovasculogenesis matures to form the circulatory system. In the adult animal, physiological angiogenesis occurs in the ovaries during the menstrual cycle and in tissue repair. Pathological angiogenesis is observed in many diseases, including inflammatory diseases, diabetic retinopathy, and cancer (2 ,3 ). Because angiogenesis plays such crucial roles in both instances, it is not surprising that this phenomenon is widely studied. Parallel to the numerous studies, there are also a large variety of angiogenic assays described (4 ).