Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions

In order to identify the nature of posttranslational modifications and enzyme functions of herpes simplex virus 1 and 2 (HSV-1 and HSV-2) proteins, it is necessary to apply both biochemical and genetic analyses. The experimental methods described in this chapter have been applied to cells cultured in vitro and infected with HSV-1 or to isolated nuclei of infected cells, to nuclear or cytoplasmic fractions, and, in some instances, to purified extracts of infected eukaryotic cells or of prokaryotic cells expressing a viral gene.