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        M13 Phage Growth and Single-Strand DNA Preparation

        互联网

        618
        Ml3 phages do not lyse their host, but are released from infected cells as the cells continue to grow and divide. Cells infected with Ml3 have a longer replication cycle than uninfected cells, and as the infection proceeds, areas of slower-growing cells can be visualized as turbid plaques on lawns of E. coli (1 ). Well-separated plaques contain cells infected with phages derived from a single transformation event and these can be picked and regrown to provide pure stocks of recombinant phage particles and DNA.
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