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        Co-staining with Thioflavin S & Carboxyfluorescein

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        Co-staining with Thioflavin S & Carboxyfluorescein

        1.) Prepare 0.01% thioflavin S (T1892 , Sigma) solution in F12+ [0.01g / 100 ml F12+]

        2.) Filter the staining solution with a 0.22 µm Millipore filter.

        3.) Incubate the staining solution and F12+ wash for approximately 30 minutes to adjust solution temperature to 37°C

        4.) Stain with thioflavin S for 15 minutes

        5.) Wash with F12+ once

        6.) Stain with carboxyfluorescein 1:200 (C1354 Molecular Probes,1mM w/DMSO) for 30 minutes

        7.) Wash with F12+ twice

        8.) View immediately under the fluorescent microscope using FITC filter.

        9.) Fix with 2% formalin for 20 to 30 minutes

        10.) Coverslip with water-soluble mounting media

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