GPCR Microspot Assays on Solid Substrates

Multiplexing and miniaturization make microarrays an attractive tool for biomolecular interaction analysis. Adequate shelf life, mechanical stability through multiple assay steps, and amenability to a microplate format for screening are core requirements for the practical large-scale implementation of microarray technology. G protein-coupled receptor (GPCR) microarrays require the co-immobilization of the receptors and their associated lipid membranes. The vulnerability of solid-supported membranes to desorption and the unique surface requirements for GPCR function provide formidable challenges for the fabrication of GPCR microarrays. The chapter describes air-stable GPCR microarrays and their utility for selectivity profiling of GPCR drugs with high fidelity.