Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPCR ORF4/GPCR GPR137 Polyclonal Antibody, Unconjugated(bs-16270R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-16270R
- 2025年10月16日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-16270R |
| 英文名称 | GPCR ORF4/GPCR GPR137 Rabbit pAb |
| 中文名称 | G蛋白偶联受体137抗体 |
| 英文别名 | C11orf4; G protein coupled receptor 137; G protein coupled receptor ORF4; G137A_HUMAN; GPR137; GPR137A; Integral membrane protein GPR137; TM7SF1L1; Transmembrane 7 superfamily member 1 like 1 protein. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GPCR ORF4/GPCR GPR137 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 46 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane. |
| 相似性 | Belongs to the GPR137 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GPR-137 is a 417 amino acid multi-pass membrane protein that belongs to the GPR-137 family. Existing as three alternatively spliced isoforms, the gene encoding GPR-137 maps to human chromosome 11q13.1. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 5.722 }}] {Lin Li. et al. ALKBH1 contributes to renal cell carcinoma progression by reducing N6-methyladenine of GPR137. EUR J CLIN INVEST. 2023 Mar;:e13986} {IHC,WB} {Human}
[IF={{ 5.5 }}] {Li Lin. et al. GPR137 inactivates Hippo signaling to promote gastric cancer cell malignancy. BIOL DIRECT. 2024 Dec;19(1):1-16} {WB} {Human}
[IF={{ 11.4 }}] {Chao Tang. et al. GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathwayJ EXP CLIN CANC RES. 2025 Jan 24;44(1):22.} {IHC} {Human}
相关实验
genome genetic and physical map data 78. IXDB http://ixdb.mpimg-berlin-dahlem.mpg.de/ Physical maps of human chromosome X 79. RHdb http://www.ebi.ac.uk/RHdb Radiation hybrid map data 80. The Unified Database http://bioinfo.weizmann.ac.il/udb
Characterization and Validation of Cre‐Driver Mouse Lines
2. (A ) Schematic representation of the CreERT2 transgene used for pronuculei injection (Feil et al., ; Indra et al., ). The pair of primers used for RT‐qPCR amplification of Cre transcripts is located in the β‐globin region between a tissue‐specific promoter X (pX
Characterization of Adrenoceptors
. Radioligand binding methods for membrane preparations and intact cells. In GPCR Signal Transduction Protocols (G.B. Willars and R.A. Challiss, eds.) pp. 1‐28. Humana Press, Totowa, N.J.
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GPCR ORF4/GPCR GPR137 Rabbit pAb(bs-16270R)-50ul/100ul/200ul
￥1180 - 2800
