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        Measurement of Nitric Oxide Formation

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        Nitric oxide (NO) is a simple gaseous monoxide that is involved in a variety of biological mechanisms in the mammalian body (1 ). NO is produced by a group of enzymes, nitric oxide synthases. One of the more important functions of NO is to regulate the tone of blood vessels (2 ). As one of the most potent vasodilators known, NO modulates the vasoconstrictive action of humoral factors, including angiotensin II (Ang II). Therefore, a well-controlled balance between Ang II and NO is required for normal hemodynamic function in all blood vessels (3 6 ).
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