3D Renal Tubule Formation Kit

3D Renal Tubule Formation Kit：

     
       The human kidney is frequently exposed to drugs and toxic compounds, which can result in nephrotoxicity. A drug’s uptake and clearance from the kidney largely relies on a subset of membrane transporters such as organic anion transporters (OAT) 1/3, organic cation transporter (OCT) 2, and multidrug and toxin extrusion proteins (MATE) 1/2. In 2D monolayer culture, primary renal epithelial cells rapidly lose the expression of OAT1/3, OCT2, and MATE1/2, limiting the accurate redictability of a drug’s effect [1]. ScienCell has developed a 3D renal tubule formation kit (3D-RTF) using human primary renal cortical epithelial cells isolated from normal adult kidney tissue, which are then embedded in Collagen I extracellular matrix. We demonstrated that renal tubule structures are formed in a robust and reproducible manner in a 3D collagenembedded model using the 3D-RTF. 3D cultured renal epithelial cells had elevated expression levels of xenobiotic transporters such as OAT1/3, and MATE1/2 (see Figure 1 and 2). The 3DRTF provides a useful in vitro tool for accurately predicting a drug’s effect on kidney function.
     人类肾脏经常接触药物和有毒化合物，可能导致肾毒性。药物从肾脏的吸收和清除主要依赖于膜转运蛋白的一个子集，如有机阴离子转运蛋白(OAT) 1/3、有机阳离子转运蛋白(OCT) 2、多药物和毒素挤压蛋白(MATE) 1/2。在二维单层培养中，原代肾上皮细胞迅速失去OAT1/3、OCT2和MATE1/2的表达，限制了药物作用[1]的准确转述。ScienCell开发了一种3D肾小管形成试剂盒(3D- rtf)，该试剂盒使用的是从正常成人肾组织中分离出的人类初级肾皮质上皮细胞，然后将其嵌入I型胶原细胞外基质中。我们用3D- rtf构建了一个3D拼贴模型，证明了肾小管结构是健壮和可复制的。3D培养的肾上皮细胞中，诸如OAT1/3和MATE1/2等异种转运蛋白的表达水平升高(见图1和图2)。

Catalog No.	3D-4110
Country of Manufacture	United States
Product Code	3D-RTF
Size/Quantity	Kit
Product Use	3D-RTF is for research use only. It is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures.
Storage	Store in -20°C .
Shipping Info	4110, 4152, 0010, and 0503 are shipped on dry ice. 4101 is shipped at room temperature. 8708-a, 8708-b, 8708-c, and 8708-d are shipped on gel ice