• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Generation and Cloning of Antigen-Specific Human T-Cells

        互联网

        644
        The ability to grow antigen-spectfic human T-cell clones in vitro has been instrumental in understanding T-cell function. A major breakthrough in T-cell culture in vitro was the discovery of the T-cell growth-inducing properties of interleukin-2 (IL-2), originally called T-cell growth factor or TCGF, by Morgan et al (1 ), who for the first time were able to grow human T-lymphocytes, isolated from human bone marrow. Shortly thereafter, Gillis and Smith (2 ) reported the cloning and long-term culture of mouse cytotoxic T-cells, using TCGF. In spite of the success of growing human T-cells in vitro, the cloning of these cells, however, turned out to be more difficult in contrast to mouse cell lines that could be maintained in culture in the presence of TCGF-containing supernatant only, long-term cultures of cloned human allo-antigen-specific T-cell lines (3 , 4 ) needed repetitive stimulation with specific allo-antigen for then growth (3 ) This led to the general assumption that antigen-specific IL-2-dependent T-cell lines and T-cell clones would lose then antigen responsiveness when propagated with IL-2 in the absence of specific antigen.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序