<center> <h1> Synchronizing Worm Cultures</h1> </center>

 

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Synchronization is based on the fact newly hatched larvae will live but not develop if deprived of a food source. An asynchronous population of embryos is isolated and then allowed to hatch in the absence of food. Once all the embryos have hatched into L1 larvae, the now synchronous population is transferred to medium with food and the population of larvae resume their growth and development.

 

Materials:

 

M9:	22 mM KH2 PO4 42 mM Na2 HPO4 85.5 mM NaCl 1 mM MgSO4
alkaline hypochlorite solution:	approx 1% NaOCl (=140 mM) 250 mM KOH
sterile culture dish or 24-well plate