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文献和实验Gene Knockout Techniques(1) The Knockout Process
liquid-grown worms.) A total of 384 worms should suffice. Try to pick the worms blindly, without regard to phenotypes, and be sure to pick worms of a range of ages in case the deletion makes them grow more slowly. (We have been using a Copas 1000N worm
ul octanol to a microfuge tube. Dip an eyebrow hair 10-15 times into octanol to initialize the hair with the solvent. (Tip4) 3. Transfer 5-8 N2 worms to #1 testing plates by a pick. Try to pick worms on the outside area of the bacteria lawn
whole-worm extracts. In principle, a protein-specific antibody is used to purify the protein of choice and its associated complex members from worm extract. This may also include RNA molecules associated with other protein components. To identify
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