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        Worm Injections

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        838

         

        Worm Injections

        pads:
        melt 2% agarose in water, keep molten at 42oC
        use 25mm x 25mm or larger microscope slides
        spread out microscope slides onto a heat resistant glass or metal tray
        using a pasteur pipet, put a small drop of agarose on to a slide and immediately cover it with another slide, pushing down
        do several slides this way
        wait a few minutes until agarose is solid and gently pry off apart the slides
        use the free (unbroken) slides as bottoms and continue until the tray is full
        when all the slides are uncovered, bake them at 80oC for several hours
        store them in a covered dish or box

        needles:
        use glass blanks made for injection needles
        use David Kopf pipet puller
        switch is on back
        take care that the filament is centered
        ours: set to 9.7

        etching needles:
        use the discarded lid of a worm plate
        put a drop of water and a drop of hydroflouric acid onto lid
        put 50 lbs. of pressure through needle as you pass the needle back and forth from acid to water
        hold needle no more than a second or so in each
        once it has begun to bubble in water pass it once more back to acid and then to water

         

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